Optimized expression and purification of myristoylated human neuronal calcium sensor 1 in E. coli

نویسندگان
چکیده

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Expression and Purification of Soluble form of Human Parathyroid Hormone (rhPTH1-34) by Trx Tag in E. coli

Background: Parathyroid Hormone (PTH) is secreted by parathyroid glands and controls the level of calcium in bones and kidney. PTH is a small polypeptide with 84 amino acids, but the first 34 amino acids of which are enough for hormone biological activity and can be used in the treatment of Osteoporosis. The expression efficiency of recombinant human parathyroid hormone rhPTH (1-34) or Teripara...

متن کامل

Optimized conditions for high-level expression and purification of recombinant human interleukin-2 in E. coli.

Interleukin-2 (IL-2), a potent cytokine has been used in anti-cancer therapy for over a decade now. IL-2, originally identified as a growth factor for T lymphocytes is a 15 kDa hydrophobic glycoprotein that induces the activation, clonal proliferation and differentiation of T and B-lymphocytes and enhances the cytotoxicity of monocytes and natural killer (NK) cells. Here, we report a simple met...

متن کامل

codon-optimized expression and purification of truncated orf2 protein of hepatitis e virus in escherichia coli

conclusions in this study, the optimized truncated orf2 protein was expressed in e. coli successfully and the highly purified protein was obtained, which can be a potential vaccine candidate and as an antigen in elisa to diagnose hev infections. results the subcloning was confirmed by pcr, restriction enzyme digestion, and dna sequencing of recombinant plasmid pet30a-orf2.2. the results obtaine...

متن کامل

Cloning, Expression and Purification of Creatininase From Pseudomonas Pseudoalkaligene KF707 in E. coli.

Creatinine amidohydrolase(EC 3.5.2.10) catalyzes the reversible conversion of creatinine to creatine. Creatininase in combination with other enzymes is used for detection of creatinine in serum and urine which is of significant value for detection of renal, muscular and thyroid functions. The aim of this study was to produce recombinant creatininase enzyme in E.coli expression system to use it ...

متن کامل

Codon-Optimized Expression and Purification of Truncated ORF2 Protein of Hepatitis E Virus in Escherichia coli

BACKGROUND Hepatitis E virus (HEV) is a causative agent of acute hepatitis among people of different age groups and has high mortality rate of up to 30% among pregnant women. Therefore, primary prevention of HEV infection is essential. OBJECTIVES The aim of this study was to obtain the highly purified truncated open reading frames 2 (ORF2) protein, which might be a future HEV vaccine candidat...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Protein Expression and Purification

سال: 2008

ISSN: 1046-5928

DOI: 10.1016/j.pep.2008.06.006